Biological Activity of Insulin-Sepharose?
نویسندگان
چکیده
منابع مشابه
Insulin-sepharose and the dynamics of insulin action.
In terms of the rate of accumulation of alpha-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro. The response to insulin-sepharose is prevented if the cells are first exposed to insulin. These observations may provide new insight into the dynamics of ...
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Four derivatives of the insuln B-chain COOH-terminal pentapeptide Arg-Gly-Phe-Phe-Tyr (B22-26) were synthesized and shown to be inactive alone. In the presence of submaximal concentrations of insulin or desoctapeptide insulin, peptides at concentrations of 10(-4) M and higher, markedly stimulated the actions of insulin on rat adipocytes including labeled glucose oxidation, activation of glycoge...
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The biological activity of Sepharose-insulin in different assays in vitro, e.g., stimulation of glucose oxidation, lipogenesis, and antilipolysis and activation of pyruvate dehydrogenase (EC 1.2.4.1) activity, has been investigated. According to amino acid analysis, between 270 and 330 mug (6.9-8.2 U) of insulin were coupled per ml of packed beads. Related to the total insulin content, 0.2-0.7%...
متن کاملActivity of select dehydrogenases with Sepharose-immobilized N6-carboxymethyl-NAD
N(6)-carboxymethyl-NAD (N(6)-CM-NAD) can be used to immobilize NAD onto a substrate containing terminal primary amines. We previously immobilized N(6)-CM-NAD onto sepharose beads and showed that Thermotoga maritima glycerol dehydrogenase could use the immobilized cofactor with cofactor recycling. We now show that Saccharomyces cerevisiae alcohol dehydrogenase, rabbit muscle L-lactate dehydrogen...
متن کاملUranyle Complexes: synthesis, evaluation of biological activity
In this research, some of the inorganic complexes of uranyl with N- donor ligandswere synthesized. Complexes were characteriezed by FT-IR and UV spectra, ¹HNMR,¹³CNMR and some physical properties. The uranyl unit (UO2) is composed of a center ofuranium atom with the charge (+6) and two oxygen atom by forming two U=O double bonds.The structure is linear (O=U=O, 180) and usually stable. So other ...
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ژورنال
عنوان ژورنال: Science
سال: 1973
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.179.4078.1142